Ambros Mashishi completed his Bachelor of Pharmacy degree in 2004 at the University of Limpopo in South Africa. He later obtained a Master of Public Health degree in 2014 from the University of Limpopo and a Post-graduate Diploma in Health Economics from the University of Cape Town in 2018.

In 2005 he joined Mankweng Hospital under the Department of Health, Limpopo Province, South Africa, to practice as a pharmacist intern and continued to practice as a community service pharmacist at Jane Furse Hospital in 2006 were he was later given a responsibility to be a senior pharmacist. Ambros took a giant step to join Ndlovu Care Group in 2014 with a key interest in HIV prevention trials and he is currently a Responsible Pharmacist at Ndlovu Care Group. He is further interested in the economics of health and firmly believe that access to healthcare should be equitable. Ambros has received several prizes for his accuracy and secure administration from diverse Trial Sponsors. He is in charge of the Research Pharmacy of Ndlovu Care Group.